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KD-Validated Anti-BID Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1971
    Western blotting analysis using anti-BID antibody (Cat#63656). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BID antibody (Cat#63656, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1971
    Western blotting analysis using anti-BID antibody (Cat#63656). BID expression in wild-type (WT) and BID shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BID antibody (Cat#63656, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1971
    Flow cytometric analysis of BID expression in HepG2 cells using anti-BID antibody (Cat#63656, 1:2,000). Green, isotype control; red, BID.
    detail
  • ICC - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1971
    Immunocytochemical staining of HepG2 cells with anti-BID antibody (Cat#63656, 1:1,000). Nuclei were stained blue with DAPI; BID was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P55957
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB6760
Calculated MW Predicted, 22 kDa, observed, 18 kDa
Gene Name BID
Aliases BID; BH3 Interacting Domain Death Agonist; BH3-Interacting Domain Death Agonist; P22 BID; Human BID Coding Sequence; Apoptic Death Agonist; Desmocollin Type 4; FP497
Immunogen Recombinant protein of human BID
Additional Information
Gene ID 637
Other Names BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, BID
Protein Information
Name BID
Function Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).
Cellular Location Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane. Note=When uncleaved, it is predominantly cytoplasmic. [BH3-interacting domain death agonist p13]: Mitochondrion membrane {ECO:0000250|UniProtKB:P70444}. Note=Associated with the mitochondrial membrane. {ECO:0000250|UniProtKB:P70444} [Isoform 3]: Cytoplasm
Tissue Location [Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). [Isoform 4]: Expressed in lung and pancreas (at protein level).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1971
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