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KD-Validated Anti-BID Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1990
    Western blotting analysis using anti-BH3 interacting domain death agonist antibody (Cat#63712). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BH3 interacting domain death agonist antibody (Cat#63712, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-BID Mouse Monoclonal Antibody AGI1990
    Western blotting analysis using anti-BH3 interacting domain death agonist antibody (Cat#63712). BH3 interacting domain death agonist expression in wild-type (WT) and BH3 interacting domain death agonist (BID) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BH3 interacting domain death agonist antibody (Cat#63712, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P55957
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1 kappa
Clone Names 24GB7035
Calculated MW Predicted, 22 kDa, observed, 19 kDa
Gene Name BID
Aliases BID; BH3 Interacting Domain Death Agonist; BH3-Interacting Domain Death Agonist; P22 BID; Human BID Coding Sequence; Apoptic Death Agonist; Desmocollin Type 4; FP497
Immunogen Recombinant protein of human BID
Additional Information
Gene ID 637
Other Names BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, BID
Protein Information
Name BID
Function Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).
Cellular Location Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane. Note=When uncleaved, it is predominantly cytoplasmic. [BH3-interacting domain death agonist p13]: Mitochondrion membrane {ECO:0000250|UniProtKB:P70444}. Note=Associated with the mitochondrial membrane. {ECO:0000250|UniProtKB:P70444} [Isoform 3]: Cytoplasm
Tissue Location [Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). [Isoform 4]: Expressed in lung and pancreas (at protein level).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1990
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