Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ovary positive   >   KD-Validated Anti-SUMO1 Mouse Monoclonal Antibody   

KD-Validated Anti-SUMO1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-SUMO1 Mouse Monoclonal Antibody AGI1997
    Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-SUMO1 Mouse Monoclonal Antibody AGI1997
    Western blotting analysis using anti-small ubiquitin like modifier 1 antibody (Cat#63746). Small ubiquitin like modifier 1 expression in wild-type (WT) and small ubiquitin like modifier 1 (SUMO1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-small ubiquitin like modifier 1 antibody (Cat#63746, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-SUMO1 Mouse Monoclonal Antibody AGI1997
    Flow cytometric analysis of Small ubiquitin like modifier 1 expression in HAP-1 cells using anti-Small ubiquitin like modifier 1 antibody (Cat#63746, 1:2,000). Green, isotype control; red, Small ubiquitin like modifier 1.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P63165
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB7175
Calculated MW Predicted, 12 kDa, observed, 75 kDa
Gene Name SUMO1
Aliases SUMO1; Small Ubiquitin Like Modifier 1; SMT3H3; SMT3C; GMP1; SUMO-1; OFC10; PIC1; UBL1; Ubiquitin-Homology Domain Protein PIC1; Small Ubiquitin-Related Modifier 1; Ubiquitin-Like Protein SMT3C; Ubiquitin-Like Protein UBL1; SMT3 Homolog 3; Sentrin; SMT3 Suppressor Of Mif Two 3 Homolog 1 (S. Cerevisiae); SMT3 Suppressor Of Mif Two 3 Homolog 1 (Yeast); SMT3 Suppressor Of Mif Two 3 Homolog 1; Ubiquitin-Like 1 (Sentrin); GAP Modifying Protein 1; GAP-Modifying Protein 1; SENP2; Smt3C; DAP1; SMT3
Immunogen Recombinant protein of human SUMO1
Additional Information
Gene ID 7341
Other Names Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1, SUMO1, SMT3C, SMT3H3, UBL1
Protein Information
Name SUMO1
Synonyms SMT3C, SMT3H3, UBL1
Function Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post- translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1 (PubMed:19223394). Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3 (PubMed:24651376).
Cellular Location Nucleus membrane. Nucleus speckle {ECO:0000250|UniProtKB:P63166}. Cytoplasm. Nucleus, PML body. Cell membrane. Nucleus. Note=Recruited by BCL11A into the nuclear body (By similarity). In the presence of ZFHX3, sequesterd to nuclear body (NB)-like dots in the nucleus some of which overlap or closely associate with PML body (PubMed:24651376) {ECO:0000250|UniProtKB:P63166, ECO:0000269|PubMed:24651376}
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 149.00
$ 499.00
Cat# AGI1997
Size:
Quantity:
Availability:
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaMexicoNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"