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> home > Products > Primary Antibodies > Antibody Collections > KD-Validated Antibodies > KD-Validated Anti-Lin-28 Homolog B Mouse Monoclonal Antibody
KD-Validated Anti-Lin-28 Homolog B Mouse Monoclonal Antibody
Mouse monoclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, ICC |
|---|---|
| Primary Accession | Q6ZN17 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Clone Names | 24GB7210 |
| Calculated MW | Predicted, 27 kDa, observed, 32 kDa |
| Gene Name | LIN28B |
| Aliases | LIN28B; Lin-28 Homolog B; CSDD2; Protein Lin-28 Homolog B; FLJ16517; Lin-28B; Lin-28 Homolog B (C. Elegans); Lin-28.2 |
| Immunogen | Recombinant protein of human LIN28B |
| Gene ID | 389421 |
|---|---|
| Other Names | Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2 |
| Name | LIN28B |
|---|---|
| Synonyms | CSDD2 |
| Function | Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'- GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let- 7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. |
| Cellular Location | Nucleus. Nucleus, nucleolus. Cytoplasm Note=Predominantly nucleolar (PubMed:22118463). In Huh7 cells, predominantly cytoplasmic, with only a subset of cells exhibiting strong nuclear staining; however, the specificity of the polyclonal antibody used in these experiments has not been not documented (PubMed:16971064). |
| Tissue Location | Expressed at high levels in the placenta and, at mucher lower, in testis and fetal liver (PubMed:16971064). Isoform 1 is only detected in placenta and in moderately and poorly differentiated hepatocellular carcinoma cells (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well- differentiated tumor tissues (at protein level). Tends to be up- regulated in triple-negative (ER-,PR-,HER2-) breast tumors, as well as in liver, ovarian, and thyroid carcinomas (PubMed:22118463) |
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$ 149.00
$ 499.00
Cat# AGI1999
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