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KD-Validated Anti-CMPK1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-CMPK1 Mouse Monoclonal Antibody AGI2013
    Western blotting analysis using anti-CMPK1 antibody (Cat#AGI2013). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CMPK1 antibody (Cat#AGI2013, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CMPK1 Mouse Monoclonal Antibody AGI2013
    Western blotting analysis using anti-CMPK1 antibody (Cat#AGI2013). CMPK1 expression in wild type (WT) and CMPK1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CMPK1 antibody (Cat#AGI2013, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CMPK1 Mouse Monoclonal Antibody AGI2013
    Flow cytometric analysis of CMPK1 expression in HepG2 cells using anti-CMPK1 antibody (Cat#AGI2013, 1:1,000). Green, isotype control; red, CMPK1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P30085
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB7965
Calculated MW Predicted, 22 kDa, observed, 20 kDa
Gene Name CMPK1
Aliases CMPK1; Cytidine/Uridine Monophosphate Kinase 1; UMP-CMP Kinase; UMP/CMP Kinase; UMP-CMPK; CMPK; Cytidine Monophosphate (UMP-CMP) Kinase 1, Cytosolic; Uridine Monophosphate/Cytidine Monophosphate Kinase; Nucleoside-Diphosphate Kinase; Deoxycytidylate Kinase; Cytidylate Kinase; DCMP Kinase; EC 2.7.4.14; UMPK; CMK; UMK; CK; Cytidine Monophosphate Kinase; Uridine Monophosphate Kinase; EC 2.7.4.6; UMP/CMPK; EC 2.7.4; UCK
Immunogen Recombinant protein of human CMPK1
Additional Information
Gene ID 51727
Other Names UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 2.7.4.14 {ECO:0000255|HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, CK {ECO:0000255|HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, Nucleoside-diphosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 2.7.4.6 {ECO:0000255|HAMAP-Rule:MF_03172}, Uridine monophosphate/cytidine monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMPK {ECO:0000255|HAMAP-Rule:MF_03172}, CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172}
Protein Information
Name CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172}
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Cellular Location Nucleus {ECO:0000255|HAMAP-Rule:MF_03172, ECO:0000269|PubMed:10462544, ECO:0000269|PubMed:11912132}. Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03172, ECO:0000269|PubMed:10462544, ECO:0000269|PubMed:11912132}. Note=Predominantly cytoplasmic, less than 15% nuclear.
Tissue Location Ubiquitously expressed.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2013
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