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KD-Validated Anti-DYNC1LI1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-DYNC1LI1 Mouse Monoclonal Antibody AGI2024
    Western blotting analysis using anti-DYNC1LI1 antibody (Cat#AGI2024). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DYNC1LI1 antibody (Cat#AGI2024, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-DYNC1LI1 Mouse Monoclonal Antibody AGI2024
    Western blotting analysis using anti-DYNC1LI1 antibody (Cat#AGI2024). DYNC1LI1 expression in wild-type (WT) and DYNC1LI1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DYNC1LI1 antibody (Cat#AGI2024, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9Y6G9
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 24GB8115
Calculated MW Predicted, 57 kDa, observed, 57 kDa
Gene Name DYNC1LI1
Aliases DYNC1LI1; Dynein Cytoplasmic 1 Light Intermediate Chain 1; DNCLI1; Dynein, Cytoplasmic, Light Intermediate Polypeptide 1; Cytoplasmic Dynein 1 Light Intermediate Chain 1; Dynein Light Intermediate Chain 1, Cytosolic 3; Dynein Light Chain A; DLIC-1; DLC-A; LIC1
Immunogen Recombinant protein of human DYNC1LI1
Additional Information
Gene ID 51143
Other Names Cytoplasmic dynein 1 light intermediate chain 1, LIC1, Dynein light chain A, DLC-A, Dynein light intermediate chain 1, cytosolic, DLIC-1, DYNC1LI1, DNCLI1
Protein Information
Name DYNC1LI1
Synonyms DNCLI1
Function Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress through the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores. Forms a functional Rab11/RAB11FIP3/dynein complex onto endosomal membrane that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC) (PubMed:20026645).
Cellular Location Cytoplasm. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Recycling endosome membrane. Note=During interphase, localized in vesicles continuously moving from peripheral sorting endosomes in the cell towards the pericentrosomal endosomal recycling compartment (ERC)
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2024
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