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KD-Validated Anti-EIF4E2 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-EIF4E2 Mouse Monoclonal Antibody AGI2027
    Western blotting analysis using anti-EIF4E2 antibody (Cat#63842). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4E2 antibody (Cat#63842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-EIF4E2 Mouse Monoclonal Antibody AGI2027
    Western blotting analysis using anti-EIF4E2 antibody (Cat#63842). EIF4E2 expression in wild type (WT) and EIF4E2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF4E2 antibody (Cat#63842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-EIF4E2 Mouse Monoclonal Antibody AGI2027
    Flow cytometric analysis of EIF4E2 expression in HepG2 cells using anti-EIF4E2 antibody (Cat#63842, 1:1,000). Green, isotype control; red, EIF4E2.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession O60573
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB8140
Calculated MW Predicted, 28 kDa, observed, 28 kDa
Gene Name EIF4E2
Aliases EIF4E2; Eukaryotic Translation Initiation Factor 4E Family Member 2; Eukaryotic Translation Initiation Factor 4E-Like 3; EIF4EL3; IF4e; 4EHP; Eukaryotic Translation Initiation Factor 4E Homologous Protein; Eukaryotic Translation Initiation Factor 4E Type 2; MRNA Cap-Binding Protein Type 3; EIF4E-Like Protein 4E-LP; EIF-4E Type 2; H4EHP; EIF4E-Like Cap-Binding Protein; MRNA Cap-Binding Protein 4EHP; EIF4E Type 2; 4E-LP
Immunogen Recombinant protein of human EIF4E2
Additional Information
Gene ID 9470
Other Names Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, h4EHP, mRNA cap-binding protein type 3, EIF4E2 {ECO:0000303|PubMed:15153109, ECO:0000312|HGNC:HGNC:3293}
Protein Information
Name EIF4E2 {ECO:0000303|PubMed:15153109, ECO:0000312|HGNC:HGNC:3293}
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation (PubMed:17368478, PubMed:22751931, PubMed:25624349, PubMed:33581076, PubMed:9582349). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity). In P-bodies, component of a complex that promotes miRNA-mediated translational repression (PubMed:28487484). Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism (PubMed:28487484, PubMed:33581076).
Cellular Location Cytoplasm. Cytoplasm, P-body
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2027
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