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KD-Validated Anti-HIBCH Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-HIBCH Mouse Monoclonal Antibody AGI2057
    Western blotting analysis using anti-HIBCH antibody (Cat#AGI2057). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIBCH antibody (Cat#AGI2057, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-HIBCH Mouse Monoclonal Antibody AGI2057
    Western blotting analysis using anti-HIBCH antibody (Cat#AGI2057). HIBCH expression in wild-type (WT) and HIBCH shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIBCH antibody (Cat#AGI2057, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q6NVY1
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB13040
Calculated MW Predicted, 43 kDa, observed, 38 kDa
Gene Name HIBCH
Aliases HIBCH; 3-Hydroxyisobutyryl-CoAHydrolase; 3-Hydroxyisobutyryl-Coenzyme A Hydrolase; 3-Hydroxyisobutyryl-CoA Hydrolase, Mitochondrial; HIB-CoA Hydrolase; HIBYL-CoA-H; EC 3.1.2.4; Testicular Tissue Protein Li 86; HIBYLCOAH
Immunogen Recombinant protein of human HIBCH
Additional Information
Gene ID 26275
Other Names 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, 3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH
Protein Information
Name HIBCH
Function Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
Cellular Location Mitochondrion.
Tissue Location Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2057
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