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KD-Validated Anti-GALE Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-GALE Mouse Monoclonal Antibody AGI2059
    Western blotting analysis using anti-GALE antibody (Cat#AGI2059). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GALE antibody (Cat#AGI2059, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-GALE Mouse Monoclonal Antibody AGI2059
    Western blotting analysis using anti-GALE antibody (Cat#AGI2059). GALE expression in wild-type (WT) and GALE shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GALE antibody (Cat#AGI2059, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q14376
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB13050
Calculated MW Predicted, 38 kDa, observed, 34 kDa
Gene Name GALE
Aliases GALE; UDP-Galactose-4-Epimerase; UDP-Glucose 4-Epimerase; SDR1E1; Short Chain Dehydrogenase/Reductase Family 1E, Member 1; UDP-N-Acetylgalactosamine 4-Epimerase; UDP-N-Acetylglucosamine 4-Epimerase; Galactose-4-Epimerase, UDP-; UDP-GalNAc 4-Epimerase; UDP-GlcNAc 4-Epimerase; Galactowaldenase; EC 5.1.3.2; Epididymis Secretory Sperm Binding Protein; UDP Galactose-4'-Epimerase; UDP-Galactose 4-Epimerase; EC 5.1.3.7; EC 5.1.3 47; THC13
Immunogen Recombinant protein of human GALE
Additional Information
Gene ID 2582
Other Names UDP-glucose 4-epimerase, 5.1.3.2, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase, 5.1.3.7, UDP-galactose 4-epimerase, GALE (HGNC:4116)
Protein Information
Name GALE (HGNC:4116)
Function Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N- acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2059
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