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KD-Validated Anti-Galactose Mutarotase Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-Galactose Mutarotase Mouse Monoclonal Antibody AGI2072
    Western blotting analysis using anti-galactose mutarotase antibody (Cat#AGI2072). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galactose mutarotase antibody (Cat#AGI2072, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Galactose Mutarotase Mouse Monoclonal Antibody AGI2072
    Western blotting analysis using anti-galactose mutarotase antibody (Cat#AGI2072). Galactose mutarotase expression in wild-type (WT) and galactose mutarotase (GALM) shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-galactose mutarotase antibody (Cat#AGI2072, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q96C23
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 24GB13160
Calculated MW Predicted, 38 kDa, observed, 36 kDa
Gene Name GALM
Aliases GALM; Galactose Mutarotase; Aldose 1-Epimerase; Aldose Mutarotase; Galactomutarotase; EC 5.1.3.3; Epididymis Secretory Sperm Binding Protein Li 63p; Galactose Enzyme Activator; HEL-S-63p; BLOCK25; GALAC4; GLAT; IBD1
Immunogen Recombinant protein of human GALM
Additional Information
Gene ID 130589
Other Names Galactose mutarotase, 5.1.3.3, Aldose 1-epimerase, GALM (HGNC:24063)
Protein Information
Name GALM (HGNC:24063)
Function Mutarotase that catalyzes the interconversion of beta-D- galactose and alpha-D-galactose during galactose metabolism (PubMed:12753898). Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway: GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase) (PubMed:30451973). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1 (PubMed:12753898). Also active on D-glucose although shows a preference for galactose over glucose (PubMed:12753898).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2072
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