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KD-Validated Anti-MRPS27 Mouse Monoclonal Antibody

Mouse monoclonal antibody

     
  • WB - KD-Validated Anti-MRPS27 Mouse Monoclonal Antibody AGI2086
    Western blotting analysis using anti-MRPS27 antibody (Cat#64855). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MRPS27 antibody (Cat#64855, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-MRPS27 Mouse Monoclonal Antibody AGI2086
    Western blotting analysis using anti-MRPS27 antibody (Cat#64855). MRPS27 expression in wild-type (WT) and MRPS27 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MRPS27 antibody (Cat#64855, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q92552
Reactivity Human
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 24GB13280
Calculated MW Predicted, 48 kDa, observed, 43 kDa
Gene Name MRPS27
Aliases MRPS27; Mitochondrial Ribosomal Protein S27; KIAA0264; MS27; 28S Ribosomal Protein S27, Mitochondrial; Small Ribosomal Subunit Protein MS27; MRP-S27; S27mt ; Mitochondrial Small Ribosomal Subunit Protein MS27; Mitochondrial 28S Ribosomal Protein S27
Immunogen Recombinant protein of human MRPS27
Additional Information
Gene ID 23107
Other Names Small ribosomal subunit protein mS27, 28S ribosomal protein S27, mitochondrial, MRP-S27, S27mt, Mitochondrial ribosomal protein S27 {ECO:0000312|HGNC:HGNC:14512}, MRPS27 (HGNC:14512), KIAA0264
Protein Information
Name MRPS27 (HGNC:14512)
Synonyms KIAA0264
Function RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis (PubMed:22841715). Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain (PubMed:22841715). Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu) (PubMed:22841715). Involved also in positive regulation of cell proliferation and tumor cell growth (PubMed:28714366).
Cellular Location Cytoplasm. Mitochondrion
Tissue Location Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level) (PubMed:28714366). Ubiquitous (PubMed:22841715)
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2086
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