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KD-Validated Anti-PDLIM7 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-PDLIM7 Rabbit Monoclonal Antibody AGI2108
    Western blotting analysis using anti-PDLIM7 antibody (Cat#AGI2108). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDLIM7 antibody (Cat#AGI2108, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-PDLIM7 Rabbit Monoclonal Antibody AGI2108
    Western blotting analysis using anti-PDLIM7 antibody (Cat#AGI2108). PDLIM7 expression in wild-type (WT) and PDLIM7 knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PDLIM7 antibody (Cat#AGI2108, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-PDLIM7 Rabbit Monoclonal Antibody AGI2108
    Flow cytometric analysis of NTHL1 expression in HAP-1 cells using anti-NTHL1 antibody (Cat# 64908, 1:2,000). Green, isotype control; red, NTHL1.
    detail
  • ICC - KD-Validated Anti-PDLIM7 Rabbit Monoclonal Antibody AGI2108
    Immunocytochemical staining of HepG2 cells with anti-PDLIM7 antibody (Cat#AGI2108, 1:1,000). Nuclei were stained blue with DAPI; PDLIM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q9NR12
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB13710
Calculated MW Predicted, 50 kDa, observed, 49 kDa
Gene Name PDLIM7
Aliases PDLIM7; PDZ And LIM Domain 7; ENIGMA; PDZ And LIM Domain 7 (Enigma); PDZ And LIM Domain Protein 7; Protein Enigma; LMP; Lim Mineralization Protein 3; LIM Mineralization Protein; LIM Domain Protein; 1110003B01Rik; LMP1; LMP3
Immunogen Recombinant protein of human PDLIM7
Additional Information
Gene ID 9260
Other Names PDZ and LIM domain protein 7, LIM mineralization protein, LMP, Protein enigma, PDLIM7, ENIGMA
Protein Information
Name PDLIM7
Synonyms ENIGMA
Function May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Colocalizes with TBX4 and TBX5 to actin filaments (By similarity).
Tissue Location Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2108
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