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KD-Validated Anti-LPCAT1 Rabbit Monoclonal Antibody

Rabbit monoclonal Antibody

     
  • WB - KD-Validated Anti-LPCAT1 Rabbit Monoclonal Antibody AGI2204
    Western blotting analysis using anti-LPCAT1 antibody (Cat#65211). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LPCAT1 antibody (Cat#65211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-LPCAT1 Rabbit Monoclonal Antibody AGI2204
    Western blotting analysis using anti-LPCAT1 antibody (Cat#65211). LPCAT1 expression in wild-type (WT) and LPCAT1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LPCAT1 antibody (Cat#65211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KD-Validated Anti-LPCAT1 Rabbit Monoclonal Antibody AGI2204
    Flow cytometric analysis of LPCAT1 expression in HepG2 cells using anti-LPCAT1 antibody (Cat#65211, 1:2,000). Green, isotype control; red, LPCAT1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q8NF37
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB180
Calculated MW Predicted, 59 kDa, observed, 55 kDa
Gene Name LPCAT1
Aliases LPCAT1; Lysophosphatidylcholine Acyltransferase 1; AGPAT10; AGPAT9; LPLAT8; AYTL2; Acetyl-CoA:Lyso-Platelet-Activating Factor Acetyltransferase; 1-Alkenylglycerophosphocholine O-Acyltransferase; 1-Alkylglycerophosphocholine O-Acetyltransferase; 1-Acylglycerophosphocholine O-Acyltransferase; 1-Acylglycerol-3-Phosphate O-Acyltransferase; Phosphonoformate Immuno-Associated Protein 3; Acetyl-CoA:Lyso-PAF Acetyltransferase; Lysophospholipid Acyltransferase 8; Lyso-PAF Acetyltransferase; LysoPC Acyltransferase 1; Acyltransferase-Like; LPC Acyltransferase 1; LysoPAFAT; FLJ12443; LPCAT-1; PFAAP3; Acyltransferase Like 2; EC 2.3.1.23; EC 2.3.1.51; EC 2.3.1.25; EC 2.3.1.67; Lpcat
Immunogen Recombinant protein of human LPCAT1
Additional Information
Gene ID 79888
Other Names Lysophosphatidylcholine acyltransferase 1, LPC acyltransferase 1, LPCAT-1, LysoPC acyltransferase 1, 2.3.1.23, 1-acylglycerol-3-phosphate O-acyltransferase, 2.3.1.51, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3, LPCAT1, AYTL2, PFAAP3
Protein Information
Name LPCAT1
Synonyms AYTL2, PFAAP3
Function Exhibits acyltransferase activity (PubMed:18156367, PubMed:21498505). Exhibits acetyltransferase activity (By similarity). Activity is calcium-independent (By similarity). Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3- phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero- 3-phosphocholine or PC) (PubMed:18156367, PubMed:21498505). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively (By similarity). Involved in platelet- activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (By similarity). May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology (By similarity). Involved in the regulation of lipid droplet number and size (PubMed:25491198).
Cellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane {ECO:0000250|UniProtKB:Q3TFD2}; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Lipid droplet. Note=May adopt a monotopic topology when embedded in the lipid monolayer of the lipid droplet, with both termini exposed to the cytoplasm.
Tissue Location Erythrocytes..
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2204
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