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KD-Validated Anti-NDUFS1 Rabbit Monoclonal Antibody

Rabbit monoclonal Antibody

     
  • WB - KD-Validated Anti-NDUFS1 Rabbit Monoclonal Antibody AGI2211
    Western blotting analysis using anti-NDUFS1 antibody (Cat#AGI2211). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFS1 antibody (Cat#AGI2211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-NDUFS1 Rabbit Monoclonal Antibody AGI2211
    Western blotting analysis using anti-NDUFS1 antibody (Cat#AGI2211). NDUFS1 expression in wild-type (WT) and NDUFS1 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFS1 antibody (Cat#AGI2211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-NDUFS1 Rabbit Monoclonal Antibody AGI2211
    Flow cytometric analysis of NDUFS1 expression in HepG2 cells using anti-NDUFS1 antibody (Cat#AGI2211, 1:2,000). Green, isotype control; red, NDUFS1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P28331
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB240
Calculated MW Predicted, 79 kDa, observed, 74 kDa
Gene Name NDUFS1
Aliases NDUFS1; NADH:Ubiquinone Oxidoreductase Core Subunit S1; NADH-Ubiquinone Oxidoreductase 75 KDa Subunit, Mitochondrial; CI-75k; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase); Complex I 75kDa Subunit 2 3NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1 (75kD) (NADH-Coenzyme Q Reductase); Mitochondrial NADH-Ubiquinone Oxidoreductase 75 KDa Subunit; Complex I, Mitochondrial Respiratory Chain, 75-KD Subunit; Complex I-75kD; EC 7.1.1.2; CI-75Kd; PRO1304; CI-75kD; MC1DN5
Immunogen A synthesized peptide derived from human Ndufs1
Additional Information
Gene ID 4719
Other Names NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, 7.1.1.2, Complex I-75kD, CI-75kD, NDUFS1
Protein Information
Name NDUFS1
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:30879903, PubMed:31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed:31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed:30879903, PubMed:31557978).
Cellular Location Mitochondrion inner membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:P15690}; Matrix side {ECO:0000250|UniProtKB:P15690}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2211
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