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KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody AGI2233
    Western blotting analysis using anti-UBE2F antibody (Cat#65322). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-UBE2F Rabbit Monoclonal Antibody AGI2233
    Western blotting analysis using anti-UBE2F antibody (Cat#65322). UBE2F expression in wild-type (WT) and UBE2F shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBE2F antibody (Cat#65322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q969M7
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB940
Calculated MW Predicted, 21 kDa; observed, 20 kDa
Gene Name UBE2F
Aliases UBE2F; Ubiquitin Conjugating Enzyme E2 F (Putative); NCE2; Ubiquitin-Conjugating Enzyme E2F (Putative); RING-Type E3 NEDD8 Transferase UBE2F; NEDD8-Conjugating Enzyme UBE2F; NEDD8 Carrier Protein UBE2F; NEDD8 Protein Ligase UBE2F; NEDD8-Conjugating Enzyme 2; Ubiquitin-Conjugating Enzyme E2 F; NEDD8 Conjugating Enzyme; EC 2.3.2.34; EC 6.3.2
Immunogen Recombinant protein of human UBE2F
Additional Information
Gene ID 140739
Other Names NEDD8-conjugating enzyme UBE2F, 2.3.2.34, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, RING-type E3 NEDD8 transferase UBE2F, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2
Protein Information
Name UBE2F
Synonyms NCE2
Function Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins (PubMed:19250909, PubMed:23201271). Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5) (PubMed:19250909, PubMed:23201271, PubMed:23300442). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B (PubMed:19250909, PubMed:23201271). Mediates neddylation of the CUL9-RBX1 complex (PubMed:38605244).
Tissue Location Widely expressed (at protein level).
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2233
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