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KD-Validated Anti-LAMB1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-LAMB1 Rabbit Monoclonal Antibody AGI2238
    Western blotting analysis using anti-LAMB1 antibody (Cat#AGI2238). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LAMB1 antibody (Cat#AGI2238, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-LAMB1 Rabbit Monoclonal Antibody AGI2238
    Western blotting analysis using anti-LAMB1 antibody (Cat#AGI2238). LAMB1 expression in wild-type (WT) and LAMB1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LAMB1 antibody (Cat#AGI2238, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-LAMB1 Rabbit Monoclonal Antibody AGI2238
    Flow cytometric analysis of LAMB1 expression in C2C12 cells using anti-LAMB1 antibody (Cat#AGI2238, 1:2,000). Green, isotype control; red, LAMB1.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P07942
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB1590
Calculated MW Predicted, 198 kDa; observed, 250 kDa
Gene Name LAMB1
Aliases LAMB1; Laminin Subunit Beta 1; Laminin Subunit Beta-1; Laminin B1 Chain; Laminin, Beta 1; CLM; Cutis Laxa With Marfanoid Phenotype; Laminin-10 Subunit Beta; Laminin-12 Subunit Beta; Laminin-1 Subunit Beta; Laminin-2 Subunit Beta; Laminin-6 Subunit Beta; Laminin-8 Subunit Beta; LIS5
Immunogen A synthesized peptide derived from human LAMB1
Additional Information
Gene ID 3912
Other Names Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1
Protein Information
Name LAMB1
Function Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface.
Cellular Location Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2238
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