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KD-Validated Anti-CCT2 beta Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CCT2 beta Rabbit Monoclonal Antibody AGI2241
    Western blotting analysis using anti-CCT2 antibody (Cat#AGI2241). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT2 antibody (Cat#AGI2241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CCT2 beta Rabbit Monoclonal Antibody AGI2241
    Western blotting analysis using anti-CCT2 antibody (Cat#AGI2241). CCT2 expression in wild-type (WT) and CCT2 knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CCT2 antibody (Cat#AGI2241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CCT2 beta Rabbit Monoclonal Antibody AGI2241
    Flow cytometric analysis of CCT2 expression in HepG2 cells using anti-CCT2 antibody (Cat#AGI2241, 1:2,000). Green, isotype control; red, CCT2.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P78371
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB1740
Calculated MW Predicted, 57 kDa; observed, 54 kDa
Gene Name CCT2
Aliases CCT2; Chaperonin Containing TCP1 Subunit 2; Cctb; Chaperonin Containing TCP1, Subunit 2 (Beta); T-Complex Protein 1 Subunit Beta; TCP-1-Beta; CCT-Beta; 99D8.1; Chaperonin Containing T-Complex Polypeptide 1, Beta Subunit; Chaperonin Containing T-Complex Polypeptide 1, Subunit 2; Chaperonin Containing T-Complex Polypeptide 1 Subunit 2; Epididymis Secretory Sperm Binding Protein Li 100n; T-Complex Protein 1, Beta Subunit; HEL-S-100n; PRO1633; CCTB
Immunogen A synthesized peptide derived from human TCP 1 beta
Additional Information
Gene ID 10576
Other Names T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT2, 99D8.1, CCTB
Protein Information
Name CCT2 {ECO:0000303|PubMed:25467444, ECO:0000312|HGNC:HGNC:1615}
Function Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:36493755, PubMed:35449234, PubMed:37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638).
Cellular Location Cytoplasm.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2241
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