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KD-Validated Anti-ECI1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-ECI1 Rabbit Monoclonal Antibody AGI2250
    Western blotting analysis using anti-ECI1 antibody (Cat#65565). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ECI1 antibody (Cat#65565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KD-Validated Anti-ECI1 Rabbit Monoclonal Antibody AGI2250
    Western blotting analysis using anti-ECI1 antibody (Cat#65565). ECI1 expression in wild-type (WT) and ECI1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ECI1 antibody (Cat#65565, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P42126
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB4285
Calculated MW Predicted, 33 kDa; observed, 28 kDa
Gene Name ECI1
Aliases ECI1; Enoyl-CoA Delta Isomerase 1; DCI; Dodecenoyl-Coenzyme A Delta Isomerase (3,2 Trans-Enoyl-Coenzyme A Isomerase); Enoyl-CoA Delta Isomerase 1, Mitochondrial; Delta(3),Delta(2)-Enoyl-CoA Isomerase; D3,D2-Enoyl-CoA Isomerase; Dodecenoyl-CoA Isomerase; Dodecenoyl-CoA Delta Isomerase (3,2 Trans-Enoyl-CoA Isomerase); 3,2-Trans-Enoyl-CoA Isomerase, Mitochondrial; Epididymis Secretory Sperm Binding Protein; 3,2 Trans-Enoyl-Coenzyme A Isomerase; 3,2 Trans-Enoyl-CoA Isomerase; 3,2-Trans-Enoyl-CoA Isomerase; Acetylene-Allene Isomerase; EC 5.3.3.8
Immunogen A synthesized peptide derived from human ECI1
Additional Information
Gene ID 1632
Other Names Enoyl-CoA delta isomerase 1, mitochondrial, 5.3.3.8, 3, 2-trans-enoyl-CoA isomerase, Delta(3), Delta(2)-enoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, ECI1, DCI
Protein Information
Name ECI1
Synonyms DCI
Function Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2- trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity) (PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length (By similarity).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P23965}
Tissue Location Expressed in liver (at protein level).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2250
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