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KD-Validated Anti-SEH1L Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-SEH1L Rabbit Monoclonal Antibody AGI2264
    Western blotting analysis using anti-Seh1L antibody (Cat#AGI2264). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Seh1L antibody (Cat#AGI2264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-SEH1L Rabbit Monoclonal Antibody AGI2264
    Western blotting analysis using anti-Seh1L antibody (Cat#AGI2264). Seh1L expression in wild-type (WT) and SEH1L shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Seh1L antibody (Cat#AGI2264, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q96EE3
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB4465
Calculated MW Predicted, 40 kDa; observed, 35 kDa
Gene Name SEH1L
Aliases SEH1L; SEH1 Like Nucleoporin; SEC13L; SEH1A; SEH1B; Seh1; Nup107-160 Subcomplex Subunit SEH1; GATOR2 Complex Protein SEH1; Sec13 Like Protein; SEC13-Like Protein; Nucleoporin Seh1; GATOR Complex Protein SEH1; SEH1-Like (S. Cerevisiae); SEH1-Like Nucleoporin; Nucleoporin SEH1; SEH1
Immunogen A synthesized peptide derived from mouse SEH1L
Additional Information
Gene ID 81929
Other Names Nucleoporin SEH1, GATOR2 complex protein SEH1, Nup107-160 subcomplex subunit SEH1, SEC13-like protein, SEH1L, SEC13L, SEH1
Protein Information
Name SEH1L
Synonyms SEC13L, SEH1
Function Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC (PubMed:15146057, PubMed:17363900). The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore (PubMed:15146057, PubMed:17363900).
Cellular Location Chromosome, centromere, kinetochore. Nucleus, nuclear pore complex. Lysosome membrane
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2264
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