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KD-Validated Anti-TPX2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TPX2 Rabbit Monoclonal Antibody AGI2286
    Western blotting analysis using anti-TPX2 antibody (Cat#AGI2286). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TPX2 antibody (Cat#AGI2286, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-TPX2 Rabbit Monoclonal Antibody AGI2286
    Western blotting analysis using anti-TPX2 antibody (Cat#AGI2286). TPX2 expression in wild-type (WT) and TPX2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TPX2 antibody (Cat#AGI2286, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9ULW0
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB4765
Calculated MW Predicted, 86 kDa; observed, 98 kDa
Gene Name TPX2
Aliases TPX2; TPX2 Microtubule Nucleation Factor; HCA519; DIL-2; P100; Hepatocellular Carcinoma-Associated Antigen 519; Targeting Protein For Xklp2; C20orf1; C20orf2; FLS353; Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2; Differentially Expressed In Cancerous And Noncancerous Lung Cells 2; Hepatocellular Carcinoma-Associated Antigen 90; Protein Fls353; DIL2; Restricted Expression Proliferation Associated Protein 100; Restricted Expression Proliferation-Associated Protein 100; TPX2, Microtubule-Associated, Homolog (Xenopus Laevis); Preferentially Expressed In Colorectal Cancer; TPX2, Microtubule-Associated Protein Homolog; Differentially Expressed In Lung Cells; TPX2, Microtubule-Associated, Homolog; Chromosome 20 Open Reading Frame 1; TPX2, Microtubule-Associated; GD:C20orf1; REPP86; HCTP4
Immunogen A synthesized peptide derived from human TPX2
Additional Information
Gene ID 22974
Other Names Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519
Protein Information
Name TPX2
Synonyms C20orf1, C20orf2, DIL2, HCA519
Function Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764, PubMed:37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation (PubMed:26165940).
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
Tissue Location Expressed in lung carcinoma cell lines but not in normal lung tissues
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2286
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