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KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody AGI2309
    Western blotting analysis using anti-GSK3 alpha/beta antibody (Cat#AGI2309). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 alpha/beta antibody (Cat#AGI2309, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody AGI2309
    Western blotting analysis using anti-GSK3 a/ antibody (Cat#AGI2309). GSK3 a/ expression in wildtype (WT) and GSK3 a/3 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-GSK3 a/ antibody(Cat#AGI2309, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody AGI2309
    Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#AGI2309, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
    detail
  • ICC - KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody AGI2309
    Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#AGI2309, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P49840/P49841
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1100
Calculated MW Predicted , 51 kDa , observed , 51,47 kDa
Gene Name GSK3A
Aliases GSK3A; Glycogen Synthase Kinase 3 Alpha; Serine/Threonine-Protein Kinase GSK3A; Glycogen Synthase Kinase-3 Alpha; EC 2.7.11.26; GSK-3 Alpha; EC 2.7.11.1; EC 2.7.11;Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
Immunogen Recombinant protein of human GSK3 alpha
Additional Information
Protein Information
Research Areas
Citations (0)
citation

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$ 149.00
$ 499.00
Cat# AGI2309
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