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KD-Validated Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody AGI2319
    Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#AGI2319). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alpha smooth muscle Actin1 antibody (Cat#AGI2319, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody AGI2319
    Western blotting analysis using anti-alpha smooth muscle Actin antibody (Cat#AGI2319). alpha smooth muscle Actin antibody expression in wild type (WT) and alpha smooth muscle Actin antibody shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-alpha smooth muscle Actin antibody (Cat#AGI2319, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody AGI2319
    Flow cytometric analysis of alpha smooth muscle Actin expression in C2C12 cells using alpha smooth muscle Actin antibody (Cat#AGI2319, 1:2,000). Green, isotype control; red, alpha smooth muscle Actin.
    detail
  • ICC - KD-Validated Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody AGI2319
    Immunocytochemical staining of C2C12 cells with alpha smooth muscle Actin antibody (Cat#AGI2319, 1:1,000). Nuclei were stained blue with DAPI; alpha smooth muscle Actin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P62736
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB600
Calculated MW Predicted, 42 kDa; Observed, 42 kDa
Gene Name ACTA2
Aliases ACTA2; Actin Alpha 2, Smooth Muscle; ACTSA; Cell Growth-Inhibiting Gene 46 Protein; Actin, Alpha 2, Smooth Muscle, Aorta; Actin, Aortic Smooth Muscle; Alpha-Cardiac Actin; Alpha-Actin-2; EC 3.6.4.-; ACTVS
Immunogen A synthesized peptide derived from human alpha smooth muscle Actin
Additional Information
Gene ID 59
Other Names Actin, aortic smooth muscle, 3.6.4.-, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, Actin, aortic smooth muscle, intermediate form, ACTA2, ACTSA, ACTVS
Protein Information
Name ACTA2
Synonyms ACTSA, ACTVS
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2319
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