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KD-Validated Anti-Acyl-CoA dehydrogenase short chain Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Acyl-CoA dehydrogenase short chain Rabbit Monoclonal Antibody AGI2342
    Western blotting analysis using anti-Acyl-CoA dehydrogenase short chain antibody (Cat#69112). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acyl-CoA dehydrogenase short chain antibody (Cat#69112, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Acyl-CoA dehydrogenase short chain Rabbit Monoclonal Antibody AGI2342
    Western blotting analysis using anti-Acyl-CoA dehydrogenase short chain antibody (Cat#69112). Acyl-CoA dehydrogenase short chain expression in wild type (WT) and acyl-CoA dehydrogenase short chain shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Acyl-CoA dehydrogenase short chain antibody (Cat#69112, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • ICC - KD-Validated Anti-Acyl-CoA dehydrogenase short chain Rabbit Monoclonal Antibody AGI2342
    Immunocytochemical staining of HeLa cells with Acyl-CoA dehydrogenase short chain antibody (Cat#69112, 1:1,000). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P16219
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1365
Calculated MW Predicted, 4 4 kDa ; Observed, 4 0 kDa
Gene Name ACADS
Aliases ACADS; Acyl-CoA Dehydrogenase Short Chain; SCAD; ACAD3; Short-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, C-2 To C-3 Short Chain; Butyryl-CoA Dehydrogenase; Mitochondrial Short-Chain Specific Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenase, C-2 To C-3 Short Chain; Epididymis Secretory Sperm Binding Protein; Unsaturated Acyl-CoA Reductase; EC 1.3.99.2; EC 1.3.8.1; EC 1.3.99
Immunogen A synthesized peptide derived from human ACADS / SCAD
Additional Information
Gene ID 35
Other Names Short-chain specific acyl-CoA dehydrogenase, mitochondrial, SCAD, 1.3.8.1, Butyryl-CoA dehydrogenase, ACADS
Protein Information
Name ACADS
Function Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl- CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed:11134486, PubMed:21237683).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:Q3ZBF6}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2342
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