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KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody AGI2356
    Western blotting analysis using anti-p150 CAF1 antibody (Cat#69311). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-p150 CAF1 antibody (Cat#69311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody AGI2356
    Western blotting analysis using anti-p150 CAF1 antibody (Cat#69311). p150 CAF1 expression in wild type (WT) and p150 CAF1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-p150 CAF1 antibody (Cat#69311, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody AGI2356
    Flow cytometric analysis of p150 CAF1 expression in HepG2 cells using p150 CAF1 antibody (Cat#69311, 1:2,000). Green, isotype control; red, p150 CAF1.
    detail
  • ICC - KD-Validated Anti-p150 CAF1 Rabbit Monoclonal Antibody AGI2356
    Immunocytochemical staining of HepG2 cells with p150 CAF1 antibody (Cat#69311, 1:1,000). Nuclei were stained blue with DAPI; p150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Midum. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q13111
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB755
Calculated MW Predicted, 107 kDa; Observed, 150 kDa
Gene Name CHAF1A
Aliases CAF1P150; CAF1B; CAF-1; CAF1; P150; Chromatin Assembly Factor I P150 Subunit; Chromatin Assembly Factor I (150 KDa); CAF-I 150 KDa Subunit; CAF-1 Subunit A; CAF-I P150; MGC71229; Hp150; Chromatin Assembly Factor 1, Subunit A (P150); CTB-50L17.7; CAF
Immunogen A synthesized peptide derived from human integrin p150 CAF1
Additional Information
Gene ID 10036
Other Names Chromatin assembly factor 1 subunit A, CAF-1 subunit A, Chromatin assembly factor I p150 subunit, CAF-I 150 kDa subunit, CAF-I p150, hp150, CHAF1A (HGNC:1910), CAF, CAF1P150
Protein Information
Name CHAF1A (HGNC:1910)
Synonyms CAF, CAF1P150
Function Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Cellular Location Nucleus. Note=DNA replication foci
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2356
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