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KD-Validated Anti-DLAT Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DLAT Rabbit Monoclonal Antibody AGI2363
    Western blotting analysis using anti-DLAT antibody (Cat#69417). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DLAT antibody (Cat#69417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-DLAT Rabbit Monoclonal Antibody AGI2363
    Western blotting analysis using anti-DLAT antibody (Cat#69417). DLAT expression in wild type (WT) and DLAT shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-DLAT antibody (Cat#69417, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-DLAT Rabbit Monoclonal Antibody AGI2363
    Flow cytometric analysis of DLAT expression in HepG2 cells using DLAT antibody (Cat#69417, 1:2,000). Green, isotype control; red, DLAT.
    detail
  • ICC - KD-Validated Anti-DLAT Rabbit Monoclonal Antibody AGI2363
    Immunocytochemical staining of HepG2 cells with DLAT antibody (Cat#69417, 1:1,000). Nuclei were stained blue with DAPI; DLAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P10515
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB805
Calculated MW Predicted, 69 kDa ; Observed, 71 kDa
Gene Name DLAT
Aliases DLAT; Dihydrolipoamide S-Acetyltransferase; PDC-E2; DLTA; E2; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate; Dehydrogenase Complex, Mitochondrial; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12; PDCE2; PBC; Dihydrolipoyllysine-Residue Acetyltransferase; EC 2.3.1
Immunogen A synthesized peptide derived from human DLAT
Additional Information
Gene ID 1737
Other Names Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, 2.3.1.12, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, PBC, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PDC-E2, PDCE2, DLAT (HGNC:2896), DLTA
Protein Information
Name DLAT (HGNC:2896)
Synonyms DLTA
Function As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety (Probable). The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:P08461}
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2363
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