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KO-Validated Anti-BCKDK Rabbit Polyclonal Antibody

Rabbit polyclonal antibody

     
  • WB - KO-Validated Anti-BCKDK Rabbit Polyclonal Antibody AGI2405
    Western blotting analysis using anti-BCKDK antibody (Cat#AGI2405). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCKDK antibody (Cat#AGI2405, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KO-Validated Anti-BCKDK Rabbit Polyclonal Antibody AGI2405
    Western blotting analysis using anti-BCKDK antibody (Cat#AGI2405). BCKDK expression in wild type (WT) and BCKDK knockout (KO) 293T cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BCKDK antibody (Cat#AGI2405, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession O14874
Reactivity Rat, Human, Mouse
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW Predicted, 46 kDa, observed, 42 kDa
Gene Name BCKDK
Aliases BCKDK; Branched Chain Keto Acid Dehydrogenase Kinase; [3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide]] Kinase, Mitochondrial; Branched-Chain Alpha-Ketoacid Dehydrogenase Kinase; BCKDH Kinase; EC 2.7.11.4; BCKDHKIN; BDK; 3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide] Kinase, Mitochondrial; Branched Chain Alpha-Ketoacid Dehydrogenase Kinase; Branched Chain Ketoacid Dehydrogenase Kinase; BCKD-Kinase; EC 2.7.11.1; EC 2.7.11; BCKDKD
Immunogen A synthesized peptide derived from human BCKDK
Additional Information
Gene ID 10295
Other Names Branched-chain alpha-ketoacid dehydrogenase kinase, BCKDH kinase, BCKDHKIN, BDK, 2.7.11.1, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, 2.7.11.4, BCKDK {ECO:0000303|PubMed:29779826, ECO:0000312|HGNC:HGNC:16902}
Protein Information
Name BCKDK {ECO:0000303|PubMed:29779826, ECO:0000312|HGNC:HGNC:16902}
Function Serine/threonine-protein kinase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with PPM1K, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched- chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle (PubMed:24449431, PubMed:29779826, PubMed:37558654). Phosphorylates and inactivates mitochondrial BCKDH complex a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Associates with the E2 component of BCKDH complex and phosphorylates BCKDHA on Ser-337, leading to conformational changes that interrupt substrate channeling between E1 and E2 and inactivates the BCKDH complex (PubMed:29779826, PubMed:37558654). Phosphorylates ACLY on Ser-455 in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and glucogenesis, respectively (PubMed:29779826). Recognizes phosphosites having SxxE/D canonical motif (PubMed:29779826).
Cellular Location Mitochondrion matrix {ECO:0000250|UniProtKB:Q00972, ECO:0000305|PubMed:24449431} Note=Detected in the cytosolic compartment of liver cells {ECO:0000250|UniProtKB:Q00972}
Tissue Location Ubiquitous.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2405
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