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KO-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KO-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody AGI2419
    Western blotting analysis using anti-Caspase 6 antibody (Cat#AGI2419). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 6 antibody (Cat#AGI2419, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KO-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody AGI2419
    Western blotting analysis using anti-caspase 6 antibody (Cat#AGI2419). Caspase 6 expression in wild type (WT) and caspase 6 (CASP6) knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 6 antibody (Cat#AGI2419, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KO-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody AGI2419
    Flow cytometric analysis of Caspase 6 expression in HAP-1 cells using Caspase 6 antibody (Cat# AGI2419, 1:2,000). Green, isotype control; red, Caspase 6.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P55212
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB465
Calculated MW Predicted, 33 kDa, observed, 33 kDa
Gene Name CASP6
Aliases CASP6; Caspase 6; CSP-6; MCH2; Caspase-6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; Mammalian Ced-3 Homologue 2; EC 3.4.22.59; Apoptotic Protease MCH-2; Apoptotic Protease Mch-2; CASPASE-6; EC 3.4.22; CASP-6
Immunogen A synthesized peptide derived from human Caspase-6
Additional Information
Gene ID 839
Other Names Caspase-6, CASP-6, CSP-6, 3.4.22.59, Apoptotic protease Mch-2, Caspase-6 subunit p18, Caspase-6 subunit p20, Caspase-6 subunit p11, Caspase-6 subunit p10, CASP6 (HGNC:1507)
Protein Information
Name CASP6 (HGNC:1507)
Function Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:19133298, PubMed:22858542, PubMed:27032039, PubMed:28864531, PubMed:30420425, PubMed:32298652, PubMed:8663580). Acts as a non- canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:11953316, PubMed:17401638, PubMed:8663580, PubMed:9463409). Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution (PubMed:11953316). Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis (PubMed:32029622). Cleaves PARK7/DJ-1 in cells undergoing apoptosis (By similarity). Involved in intrinsic apoptosis by mediating cleavage of RIPK1 (PubMed:22858542). Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026). Cleaves phospholipid scramblase proteins XKR4 and XKR9 (By similarity). In addition to apoptosis, involved in different forms of programmed cell death (PubMed:32298652). Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity (PubMed:32298652). PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis (PubMed:32298652). Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652). Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity). Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
Cellular Location Cytoplasm. Nucleus
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2419
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