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KO-Validated Anti-Ribosomal Protein SA Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KO-Validated Anti-Ribosomal Protein SA Rabbit Monoclonal Antibody AGI2429
    Western blotting analysis using anti-ribosomal protein SA antibody (Cat#71214). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ribosomal protein SA antibody (Cat#71214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KO-Validated Anti-Ribosomal Protein SA Rabbit Monoclonal Antibody AGI2429
    Western blotting analysis using anti-ribosomal protein SA antibody (Cat#71214). Ribosomal protein SA expression in wild type (WT) and ribosomal protein SA (RPSA) knockout (KO) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ribosomal protein SA antibody (Cat#71214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KO-Validated Anti-Ribosomal Protein SA Rabbit Monoclonal Antibody AGI2429
    Flow cytometric analysis of Ribosomal protein SAc expression in HepG2 cells using anti-Ribosomal protein SA antibody (Cat#71214, 1:2,000). Green, isotype control; red, Ribosomal protein SA.
    detail
  • ICC - KO-Validated Anti-Ribosomal Protein SA Rabbit Monoclonal Antibody AGI2429
    Immunocytochemical staining of HepG2 cells with anti-Ribosomal protein SA antibody (Cat#71214, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein SA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P08865
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2950
Calculated MW Predicted, 33 kDa; observed, 42 kDa
Gene Name RPSA
Aliases RPSA; Ribosomal Protein SA; 37LRP; LAMR1; LRP; P40; US2; SA; Laminin Receptor 1 (67kD, Ribosomal Protein SA); Multidrug Resistance-Associated Protein MGr1-Ag; Colon Carcinoma Laminin-Binding Protein; Laminin-Binding Protein Precursor P40; Small Ribosomal Subunit Protein US2; 37/67 KDa Laminin Receptor; 40S Ribosomal Protein SA; 67 KDa Laminin Receptor; NEM/1CHD4; LBP/P40; LRP/LR; LAMBR; 67LR; LamR; 37 KDa Laminin Receptor Precursor; 37 KDa Laminin Receptor; Laminin Receptor 1; ICAS; LBP
Immunogen A synthesized peptide derived from human 67kDa Laminin Receptor
Additional Information
Gene ID 3921
Other Names Small ribosomal subunit protein uS2 {ECO:0000255|HAMAP-Rule:MF_03016, ECO:0000303|PubMed:24524803}, 37 kDa laminin receptor precursor {ECO:0000255|HAMAP-Rule:MF_03016}, 37LRP {ECO:0000255|HAMAP-Rule:MF_03016}, 37/67 kDa laminin receptor {ECO:0000255|HAMAP-Rule:MF_03016}, LRP/LR {ECO:0000255|HAMAP-Rule:MF_03016}, 40S ribosomal protein SA, 67 kDa laminin receptor {ECO:0000255|HAMAP-Rule:MF_03016}, 67LR {ECO:0000255|HAMAP-Rule:MF_03016}, Colon carcinoma laminin-binding protein, Laminin receptor 1 {ECO:0000255|HAMAP-Rule:MF_03016}, LamR {ECO:0000255|HAMAP-Rule:MF_03016}, Laminin-binding protein precursor p40 {ECO:0000255|HAMAP-Rule:MF_03016}, LBP/p40 {ECO:0000255|HAMAP-Rule:MF_03016}, Multidrug resistance-associated protein MGr1-Ag, NEM/1CHD4, RPSA {ECO:0000255|HAMAP-Rule:MF_03016}, LAMBR, LAMR1
Protein Information
Name RPSA {ECO:0000255|HAMAP-Rule:MF_03016}
Synonyms LAMBR, LAMR1
Function Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA- precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.
Cellular Location Cell membrane. Cytoplasm. Nucleus {ECO:0000255|HAMAP-Rule:MF_03016}. Note=67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity) Colocalizes with PPP1R16B in the cell membrane. {ECO:0000255|HAMAP- Rule:MF_03016}
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$ 149.00
$ 499.00
Cat# AGI2429
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