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KO-Validated Anti-GSTO1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KO-Validated Anti-GSTO1 Rabbit Monoclonal Antibody AGI2431
    Western blotting analysis using anti-glutathione S-transferase omega 1 antibody (Cat#71218). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glutathione S-transferase omega 1 antibody (Cat#71218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • WB - KO-Validated Anti-GSTO1 Rabbit Monoclonal Antibody AGI2431
    Western blotting analysis using anti-glutathione S-transferase omega 1 antibody (Cat#71218). Glutathione S-transferase omega 1 expression in wild type (WT) and glutathione S-transferase omega 1 (GSTO1) knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-glutathione S-transferase omega 1 antibody (Cat#71218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
    detail
  • FC - KO-Validated Anti-GSTO1 Rabbit Monoclonal Antibody AGI2431
    Flow cytometric analysis of Glutathione S-transferase omega 1 expression in HepG2 cells using anti-Glutathione S-transferase omega 1 antibody (Cat#71218, 1:2,000). Green, isotype control; red, Glutathione S-transferase omega 1.
    detail
  • ICC - KO-Validated Anti-GSTO1 Rabbit Monoclonal Antibody AGI2431
    Immunocytochemical staining of HepG2 cells with anti-Glutathione S-transferase omega 1 antibody (Cat#71218, 1:1,000). Nuclei were stained blue with DAPI; Glutathione S-transferase omega 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P78417
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3120
Calculated MW Predicted, 28 kDa; observed, 28 kDa
Gene Name GSTO1
Aliases GSTO1; Glutathione S-Transferase Omega 1; GSTTLp28; P28; Glutathione-Dependent Dehydroascorbate Reductase; Glutathione S-Transferase Omega 1-1; Glutathione S-Transferase Omega-1; S-(Phenacyl)Glutathione Reductase; Monomethylarsonic Acid Reductase; MMA(V) Reductase; EC 2.5.1.18; GSTO 1-1; SPG-R; Epididymis Secretory Protein Li 21; Glutathione-S-Transferase Like; EC 1.20.4.2; EC 1.8.5.1; HEL-S-21; GSTTLP28; GSTO-1
Immunogen A synthesized peptide derived from human GSTO1
Additional Information
Gene ID 9446
Other Names Glutathione S-transferase omega-1, GSTO-1, 2.5.1.18, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, 1.8.5.1, Monomethylarsonic acid reductase, MMA(V) reductase, 1.20.4.2, S-(Phenacyl)glutathione reductase, SPG-R, GSTO1, GSTTLP28
Protein Information
Name GSTO1
Synonyms GSTTLP28
Function Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Also has glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
Cellular Location Cytoplasm, cytosol.
Tissue Location Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart Lowest expression in brain, placenta and lung
Research Areas
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$ 149.00
$ 499.00
Cat# AGI2431
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