Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody   

KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody AGI2435
    Western blotting analysis using anti-STK3 antibody (Cat#AGI2435). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STK3 antibody (Cat#AGI2435, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
    detail
  • WB - KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody AGI2435
    Western blotting analysis using anti-STK3 antibody (Cat#AGI2435). STK3 expression in wild type (WT) and STK3 knockout (KO) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-STK3 antibody (Cat#AGI2435, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody AGI2435
    Flow cytometric analysis of STK3 expression in HepG2 cells using anti-STK3 antibody (Cat#AGI2435, 1:2,000). Green, isotype control; red, STK3.
    detail
  • ICC - KO-Validated Anti-Serine/Threonine Kinase 3 Rabbit Monoclonal Antibody AGI2435
    Immunocytochemical staining of HepG2 cells with anti-STK3 antibody (Cat#AGI2435, 1:1,000). Nuclei were stained blue with DAPI; STK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q13188
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB3265
Calculated MW Predicted, 56 kDa; observed, 56 kDa
Gene Name STK3
Aliases STK3; Serine/Threonine Kinase 3; MST2; KRS1; Serine/Threonine Kinase 3 (Ste20, Yeast Homolog); Mammalian STE20-Like Protein Kinase 2; Serine/Threonine-Protein Kinase Krs-1; Serine/Threonine-Protein Kinase 3; STE20-Like Kinase MST2; EC 2.7.11.1; MST-2; Serine/Threonine Kinase 3 (STE20 Homolog, Yeast); Epididymis Secretory Sperm Binding Protein; Hippo (Drosophila) Homolog; Hippo Homolog; KB-1458E12.1; EC 2.7.11
Immunogen A synthesized peptide derived from human STK3
Additional Information
Gene ID 6788
Other Names Serine/threonine-protein kinase 3, 2.7.11.1, Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, Serine/threonine-protein kinase 3 36kDa subunit, MST2/N, Serine/threonine-protein kinase 3 20kDa subunit, MST2/C, STK3 (HGNC:11406)
Protein Information
Name STK3 (HGNC:11406)
Function Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed:11278283, PubMed:8566796, PubMed:8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714, PubMed:29063833, PubMed:30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:15688006, PubMed:16930133, PubMed:23972470, PubMed:28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed:21076410, PubMed:21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed:21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed:20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed:19525978). Phosphorylates MOBKL1B on 'Thr- 74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed:18328708, PubMed:18362890).
Cellular Location Cytoplasm. Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=The caspase-cleaved form cycles between nucleus and cytoplasm (PubMed:11278283, PubMed:19525978) Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (PubMed:19525978).
Tissue Location Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 149.00
$ 499.00
Cat# AGI2435
Size:
Quantity:
Availability:
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaMexicoNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"