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CD50 / ICAM-3 Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM505 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, FC |
|---|---|
| Primary Accession | P32942 |
| Other Accession | 3385, 354563 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2b, kappa |
| Clone Names | SPM505 |
| Calculated MW | 110-160kDa |
| Gene ID | 3385 |
|---|---|
| Other Names | Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, CD50, ICAM3 |
| Application Note | IHC-P~~N/A IF~~1:50~200 FC~~1:10~50 |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD50 / ICAM-3 Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ICAM3 |
|---|---|
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha- L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). |
| Cellular Location | Membrane; Single-pass type I membrane protein. |
| Tissue Location | Leukocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
References
LeukocyteTyping V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995)p. 1546-1547, 1578-1579. | C.L. Holness, et al, (1995) J Biol Chem 270: 877-884. | Y. van Kooyk,et al, (1996) J Exp Med 183:1247-1252. | Leukocyte TypingVI (T. Kishimoto, et al, eds.) GarlandPublishing, Inc., New York (1997) p.403-409
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