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TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM357 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC |
|---|---|
| Primary Accession | P01135 |
| Other Accession | 7039, 170009 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | SPM357 |
| Calculated MW | ~6kDa |
| Gene ID | 7039 |
|---|---|
| Other Names | Protransforming growth factor alpha, Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, TGFA |
| Application Note | IF~~1:50~200 FC~~1:10~50 |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | TGF-alpha (Transforming Growth Factor alpha) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TGFA |
|---|---|
| Function | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. |
| Cellular Location | [Transforming growth factor alpha]: Secreted, extracellular space |
| Tissue Location | Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
References
Kobrin MS et. al. J Bio Chem, 1986, 261:14414-9. | Kudlow JE et. al. Endocrinology, 1987, 121(4):1577-9. | Kobrin MS et. al. Endocrinology, 1987, 121(4):1412-6. | Kudlow JE et. al. J Bio Chem, 1989, 264(7):3880-3 |
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