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Thymidylate Synthase (5-FU Resistance Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone SPM453 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, FC |
|---|---|
| Primary Accession | P04818 |
| Other Accession | 7298, 369762 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | SPM453 |
| Calculated MW | 36kDa |
| Gene ID | 7298 |
|---|---|
| Other Names | Thymidylate synthase, TS, TSase, 2.1.1.45, TYMS, TS |
| Application Note | IHC-P~~N/A IF~~1:50~200 FC~~1:10~50 |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Thymidylate Synthase (5-FU Resistance Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TYMS (HGNC:12441) |
|---|---|
| Synonyms | TS |
| Function | Catalyzes the reductive methylation of 2'-deoxyuridine 5'- monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1- carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway. |
| Cellular Location | Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
References
Johnston PG, et. al. Cancer Research, 1991, 51(24):6668-76. | Johnston PG, et. al. Cancer Research, 1992, 52(16):4306-12
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