CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone FR4A5 ]
|Application ||IF, FC|
|Other Accession||2526, 654379|
|Isotype||Mouse / IgM, kappa|
|Calculated MW||~220kDa (Glycoprotein)|
|Other Names||Alpha-(1, 3)-fucosyltransferase 4, 2.4.1.-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A|
|Storage||Store at 2 to 8°C.Antibody is stable for 24 months.|
|Precautions||CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||[Isoform Short]: Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:29593094, PubMed:1702034, PubMed:1716630). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6- sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:29593094, PubMed:1716630). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:1716630, PubMed:11278338).|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Single- pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi|
|Tissue Location||[Isoform Short]: Expressed at low levels in bone marrow-derived mesenchymal stem cells.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin’s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Bettelheim, P., et al., Cluster Report: CD15, in : Knapp, W., et al. (eds), Leucocyte Typing IV, Oxford Univ. Press, pp 798-799. | Hogg, N., et al., Myeloid antigens: new and previously defines clusters, in: McMichae, A.J., et al (eds), Leucocyte Typing III, Oxford Univ. Press, pp 576-602
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.