Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
CD53 (TSPAN25) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone 63-5A3 ]
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, FC |
|---|---|
| Primary Accession | P19397 |
| Other Accession | 963, 443057 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2b, kappa |
| Clone Names | 63-5A3 |
| Calculated MW | 33-55kDa |
| Gene ID | 963 |
|---|---|
| Other Names | Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD53, CD53, MOX44, TSPAN25 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | CD53 (TSPAN25) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD53 |
|---|---|
| Synonyms | MOX44, TSPAN25 |
| Function | Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:32974937, PubMed:35767951). Plays a role in the activation of monocytes and B-cells (PubMed:8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Also promotes, in B-cells, the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed:28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Also mediates metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity). |
| Cellular Location | Cell membrane. Cell junction {ECO:0000250|UniProtKB:Q61451}. Membrane; Multi-pass membrane protein. Synapse. Note=Concentrates in localized microdomains along the plasma membrane at the contact sites between cells of fused myotubes. {ECO:0000250|UniProtKB:Q61451} |
| Tissue Location | B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
References
Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989 | Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. | Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997. | Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716. | Mannion BA et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin (CD49d/CD29). J Immunol 1996, 157(5):2039-2047. | Carmo AM et al. Association of the transmembrane 4-superfamily molecule CD53 with a tyrosine phosphatase activity. Eur J Immunol 1995, 25(7):2090-2095. | Mollinedo F et al. Recurrent infectious diseases in human CD53 deficiency. Clin Diagn Lab Immunol 1997, 4(2):229-231
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
