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Anti-Factor XIIIa Antibody

Mouse Monoclonal Antibody

     
  • IHC-P - Anti-Factor XIIIa Antibody AH13208-20
    Formalin-fixed, paraffin-embedded human Histiocytoma stained with Factor XIIIa Monoclonal Antibody (F13A1/1448).
    detail
  • WB - Anti-Factor XIIIa Antibody AH13208-20
    Western Blot Analysis of Recombinant Protein and HeLa Cell Lysate using Factor XIIIa Monoclonal Antibody (F13A1/1448).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P00488
Other Accession 335513
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG2b, kappa
Clone Names F13A1/1448
Calculated MW 83268 Da
Additional Information
Gene ID 2162
Other Names Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; Coagulation factor XIIIa; F13A; F13a1; Factor XIIIA; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase A chain; Transglutaminase. plasma
Application Note ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); ,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml);,Western Blotting (0.5-1.0ug/ml);,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-Factor XIIIa Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name F13A1
Synonyms F13A
Function Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Cellular Location Cytoplasm. Secreted. Note=Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma
Research Areas
Citations (0)
citation

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Background

It recognizes a protein of 83kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.

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$ 219.00
$ 499.00
Cat# AH13208-20
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