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Anti-CD99 / MIC2 Antibody
Recombinant Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, IF, FC |
|---|---|
| Primary Accession | P14209 |
| Other Accession | 653349 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit / IgG, kappa |
| Clone Names | MIC2/1495R |
| Calculated MW | 18848 Da |
| Gene ID | 4267 |
|---|---|
| Other Names | 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2 |
| Application Note | Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); ,Immunohistology (Formalin-fixed) (5-10ug/ml for 30 min at Room Temp),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-CD99 / MIC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD99 |
|---|---|
| Synonyms | MIC2, MIC2X, MIC2Y |
| Function | Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity). |
| Cellular Location | Membrane; Single-pass type I membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
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