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Anti-CD22 / BL-CAM Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC, E |
|---|---|
| Primary Accession | P20273 |
| Other Accession | 579691 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1, kappa |
| Clone Names | BLCAM/1795 |
| Calculated MW | 95348 Da |
| Gene ID | 933 |
|---|---|
| Other Names | B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14 |
| Application Note | ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA);,Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); ,Western Blotting (0.5-1.0ug/ml);,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-CD22 / BL-CAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CD22 {ECO:0000303|PubMed:1691828, ECO:0000312|HGNC:HGNC:1643} |
|---|---|
| Function | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6- linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | B-lymphocytes. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
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