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Anti-Cytokeratin, Acidic (Type I or LMW) Antibody
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, FC |
|---|---|
| Primary Accession | Q7Z794 |
| Other Accession | 334989 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG1 |
| Clone Names | KRTL/1377 |
| Calculated MW | 61901 Da |
| Gene ID | 374454 |
|---|---|
| Other Names | K1B; KRT1B; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B |
| Application Note | Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Format | 200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Anti-Cytokeratin, Acidic (Type I or LMW) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | KRT77 |
|---|---|
| Synonyms | KRT1B |
| Tissue Location | Expressed exclusively in skin. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
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