H2AFY antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O75367 |
Other Accession | NM_001040158, NP_001035248 |
Reactivity | Human, Mouse, Rat, Rabbit, Pig, Horse, Yeast, Bovine, Guinea Pig, Dog |
Predicted | Human, Mouse, Rat, Rabbit, Pig, Chicken, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 39kDa |
Gene ID | 9555 |
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Alias Symbol | H2A.y, H2A/y, H2AF12M, H2AFJ, MACROH2A1.1, mH2A1, macroH2A1.2 |
Other Names | Core histone macro-H2A.1, Histone macroH2A1, mH2A1, Histone H2A.y, H2A/y, Medulloblastoma antigen MU-MB-50.205, H2AFY, MACROH2A1 |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-H2AFY antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | H2AFY antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MACROH2A1 (HGNC:4740) |
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Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription (PubMed:12718888, PubMed:15621527, PubMed:16428466). Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template (PubMed:15897469). Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability (PubMed:15897469). DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation (PubMed:15897469). Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes (PubMed:12718888, PubMed:16428466). Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin (PubMed:16428466, PubMed:16107708). |
Cellular Location | Nucleus. Chromosome. Note=Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin (PubMed:15621527, PubMed:15897469, PubMed:9634239). Recruited to DNA damage sites in an APLF-dependent manner (PubMed:21211722, PubMed:29905837). |
Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
References
Lee Y.,et al.Biochim. Biophys. Acta 1399:73-77(1998).
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmutz J.,et al.Nature 431:268-274(2004).
Behrends U.,et al.Int. J. Cancer 106:244-251(2003).
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