Rab5a antibody - middle region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9CQD1 |
Other Accession | NM_025887, NP_080163 |
Reactivity | Human, Mouse, Rat, Rabbit, Pig, Sheep, Horse, Bovine, Guinea Pig, Dog |
Predicted | Human, Mouse, Rat, Pig, Chicken, Sheep, Bovine, Guinea Pig, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 24kDa |
Gene ID | 271457 |
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Alias Symbol | 2410015H04Rik, AI663973, AU021172 |
Other Names | Ras-related protein Rab-5A, Rab5a, nnyRab5a |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-Rab5a antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | Rab5a antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Rab5a |
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Synonyms | nnyRab5a |
Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (By similarity). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (PubMed:18425118). |
Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P20339}; Lipid-anchor {ECO:0000250|UniProtKB:P20339}; Cytoplasmic side {ECO:0000250|UniProtKB:P18066}. Early endosome membrane {ECO:0000250|UniProtKB:P20339}; Lipid-anchor {ECO:0000250|UniProtKB:P20339}. Melanosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P20339}. Cell projection, ruffle {ECO:0000250|UniProtKB:P18066}. Membrane {ECO:0000250|UniProtKB:P20339}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:P20339}. Cytoplasmic vesicle, phagosome membrane. Endosome membrane {ECO:0000250|UniProtKB:P20339}. Note=Enriched in stage I melanosomes Alternates between membrane-bound and cytosolic forms {ECO:0000250|UniProtKB:P20339} |
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Provided below are standard protocols that you may find useful for product applications.
References
Kuroda T.S.,et al.J. Biol. Chem. 277:9212-9218(2002).
Itoh T.,et al.Genes Cells 11:1023-1037(2006).
Carninci P.,et al.Science 309:1559-1563(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (APR-2007) to UniProtKB.
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