|Other Accession||NM_004869, NP_004860|
|Reactivity||Human, Rat, Rabbit, Pig, Horse, Bovine, Guinea Pig, Dog|
|Predicted||Human, Rabbit, Pig, Bovine, Guinea Pig, Dog|
|Alias Symbol||MIG1, SKD1, SKD1B, VPS4-2|
|Other Names||Vacuolar protein sorting-associated protein 4B, 188.8.131.52, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-VPS4B antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||VPS4B antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).|
|Cellular Location||Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Scheuring S.,et al.J. Mol. Biol. 312:469-480(2001).
Beyer A.,et al.Gene 305:47-59(2003).
Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Kim J.W.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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