|Other Accession||NM_022149, NP_071432|
|Other Names||Melanoma-associated antigen F1, MAGE-F1 antigen, MAGEF1|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 ul of distilled water. Final anti-MAGEF1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||MAGEF1 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Enhances ubiquitin ligase activity of RING-type zinc finger- containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair (PubMed:29225034).|
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Provided below are standard protocols that you may find useful for product applications.
Stone B.,et al.Gene 267:173-182(2001).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Chomez P.,et al.Cancer Res. 61:5544-5551(2001).
Doyle J.M.,et al.Mol. Cell 39:963-974(2010).
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