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SPHK1 antibody - middle region

Rabbit Polyclonal Antibody

     
  • WB - SPHK1 antibody - middle region AI14965

    WB Suggested Anti-SPHK1 Antibody Titration: 1.0 μg/ml
    Positive Control: Fetal Lung
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q9NYA1
Other Accession NM_001142601, NP_001136073
Reactivity Human, Mouse, Rat, Rabbit, Pig, Goat, Horse, Bovine, Guinea Pig, Dog
Predicted Human, Mouse, Rat, Rabbit, Chicken, Horse, Bovine, Guinea Pig, Dog
Host Rabbit
Clonality Polyclonal
Calculated MW 42kDa
Additional Information
Gene ID 8877
Alias Symbol SPHK
Other Names Sphingosine kinase 1, SK 1, SPK 1, 2.7.1.91, SPHK1, SPHK, SPK
Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage Add 50 ul of distilled water. Final anti-SPHK1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
PrecautionsSPHK1 antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1 (HGNC:11240)
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo- dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:20577214, PubMed:23602659, PubMed:29662056, PubMed:24929359, PubMed:11923095). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF- kappa-B in response to TNF signaling leading to IL17 secretion (PubMed:20577214). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (PubMed:23935096). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:28049734, PubMed:24929359). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698).
Cellular Location Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse {ECO:0000250|UniProtKB:Q8CI15}. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner (PubMed:19854831). Binds to membranes containing negatively charged lipids but not neutral lipids (PubMed:24929359). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (By similarity) {ECO:0000250|UniProtKB:Q8CI15, ECO:0000269|PubMed:19854831, ECO:0000269|PubMed:24929359}
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level) (PubMed:29662056).
Research Areas
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References

Melendez A.J.,et al.Gene 251:19-26(2000).
Nava V.E.,et al.FEBS Lett. 473:81-84(2000).
Pitson S.M.,et al.Biochem. J. 350:429-441(2000).
Van Veldhoven P.P.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 389.00
Cat# AI14965
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