|Alias Symbol||SPAM1, HYAL3, PH20,|
|Other Names||Hyaluronidase PH-20, Hyal-PH20, 188.8.131.52, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, SPAM1, HYAL3, PH20|
|Format||Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.|
|Reconstitution & Storage||Add 50 &mu, l of distilled water. Final Anti-SPAM1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.|
|Precautions||SPAM1 Antibody - middle region is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
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Provided below are standard protocols that you may find useful for product applications.
Lin Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075(1993).
Gmachl M.,et al.FEBS Lett. 336:545-548(1993).
Jones M.H.,et al.Genomics 29:796-800(1995).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
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