GSPT1 Antibody - N-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P15170 |
Other Accession | NM_001130006, NP_001123478 |
Reactivity | Human, Rat, Rabbit, Pig, Bovine, Dog |
Predicted | Human, Rat, Rabbit, Pig, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 70kDa |
Gene ID | 2935 |
---|---|
Alias Symbol | 551G9.2, ETF3A, FLJ38048, FLJ39067, GST1, eRF3a |
Other Names | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog, GSPT1, ERF3A |
Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reconstitution & Storage | Add 50 ul of distilled water. Final anti-GSPT1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
Precautions | GSPT1 Antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GSPT1 |
---|---|
Synonyms | ERF3A |
Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:2511002, PubMed:15987998, PubMed:19417105, PubMed:27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A- site (PubMed:16777602, PubMed:27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
References
Hoshino S.,et al.EMBO J. 8:3807-3814(1989).
Loftus B.J.,et al.Genomics 60:295-308(1999).
Martin J.,et al.Nature 432:988-994(2004).
Yamashita A.,et al.Genes Dev. 23:1091-1105(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.