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APLF Antibody - C-terminal region
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q8IW19 |
| Other Accession | NM_173545, NP_775816 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine |
| Predicted | Human, Mouse, Rat, Rabbit, Pig, Horse, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 57kDa |
| Gene ID | 200558 |
|---|---|
| Alias Symbol | APFL, C2orf13, FLJ16593, MGC47799, PALF, Xip1 |
| Other Names | Aprataxin and PNK-like factor, 4.2.99.18, Apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, XRCC1-interacting protein 1, APLF, C2orf13, PALF, XIP1 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 50 ul of distilled water. Final anti-APLF antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | APLF Antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | APLF {ECO:0000303|PubMed:17353262, ECO:0000312|HGNC:HGNC:28724} |
|---|---|
| Function | Histone chaperone involved in single-strand and double-strand DNA break repair (PubMed:17353262, PubMed:17396150, PubMed:21211721, PubMed:21211722, PubMed:29905837, PubMed:30104678). Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions (PubMed:17353262, PubMed:17396150, PubMed:21211721, PubMed:30104678). Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1 (PubMed:21211722, PubMed:29905837, PubMed:30104678). Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro (PubMed:17353262, PubMed:17396150). Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage (PubMed:17353262, PubMed:17396150). Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed:21211721, PubMed:23689425). Also acts as a negative regulator of cell pluripotency by promoting histone exchange (By similarity). Required for the embryo implantation during the epithelial to mesenchymal transition in females (By similarity). |
| Cellular Location | Nucleus. Chromosome. Cytoplasm, cytosol. Note=Localizes to DNA damage sites (PubMed:18172500, PubMed:18474613, PubMed:21211721, PubMed:21211722, PubMed:23689425). Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (PubMed:18172500) |
Citations (0)
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Kanno S.,et al.EMBO J. 26:2094-2103(2007).
Bekker-Jensen S.,et al.J. Biol. Chem. 282:19638-19643(2007).
Iles N.,et al.Mol. Cell. Biol. 27:3793-3803(2007).
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$ 389.00
Cat# AI15209
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