NR5A2 / LRH-1 Antibody (C-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P |
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Primary Accession | O00482 |
Reactivity | Human, Monkey, Pig, Sheep, Horse, Bovine, Dog |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 61kDa |
Dilution | IHC-P (7 µg/ml) |
Gene ID | 2494 |
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Other Names | Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1, NR5A2, B1F, CPF, FTF |
Target/Specificity | Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Reconstitution & Storage | Long term: -70°C; Short term: +4°C |
Precautions | NR5A2 / LRH-1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NR5A2 |
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Synonyms | B1F, CPF, FTF |
Function | Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti- inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity). |
Cellular Location | Nucleus. |
Tissue Location | Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
References
Li M.,et al.J. Biol. Chem. 273:29022-29031(1998).
Li M.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Nitta M.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:6660-6665(1999).
Zhang C.K.,et al.Gene 273:239-249(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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