BMI1 / PCGF4 Antibody (aa252-264)

Anti-BMI-1 antibody IHC of human skeletal muscle.
Anti-Bmi1 Antibody - Immunofluorescence Microscopy.

SPECIFICATION
CITATIONS
PROTOCOLS
BACKGROUND

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC-P, IF, ICC, E
Primary Accession	P35226
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Calculated MW	36949 Da
Dilution	ELISA (1:5000-1:30000), IHC-P (2.5 µg/ml), WB (1:500-1:3000)

Additional Information
Gene ID	100532731;648
Other Names	Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51
Target/Specificity	Amino acids 252-264 of human Bmi1 protein.
Reconstitution & Storage	Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
Precautions	BMI1 / PCGF4 Antibody (aa252-264) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	BMI1
Synonyms	PCGF4, RNF51
Function	Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:26151332, PubMed:27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:21772249, PubMed:26151332).
Cellular Location	Nucleus. Cytoplasm
Volume	50 µl

Research Areas

Primary Antibodies

Citations (0)

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

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Goat Polyclonal Antibody

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