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SAFB1 / SAFB Antibody (aa345-357, clone 6F7)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, IP |
|---|---|
| Primary Accession | Q15424 |
| Reactivity | Human, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 6F7 |
| Calculated MW | 103kDa |
| Dilution | IHC-P (5 µg/ml) |
| Gene ID | 6294 |
|---|---|
| Other Names | Scaffold attachment factor B1, SAF-B, SAF-B1, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein, SAFB, HAP, HET, SAFB1 |
| Target/Specificity | A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein. |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | SAFB1 / SAFB Antibody (aa345-357, clone 6F7) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SAFB |
|---|---|
| Synonyms | HAP, HET, SAFB1 |
| Function | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed:9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed:12660241). Thereby acts as a negative regulator of cell proliferation (PubMed:12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). |
| Cellular Location | Nucleus |
| Tissue Location | Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines |
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Provided below are standard protocols that you may find useful for product applications.
Background
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.
References
Oesterreich S.,et al.J. Cell. Biochem. 67:275-286(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (JUL-2009) to UniProtKB.
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